Staphylococcus saprophyticus lipase gene database comprises of lipase gene sequences and translation protein details. Lipase gene consists of Triacylglycerol esterase/lipase EstA domain and alpha/beta hydrolase fold which aid in lipid transport and metabolism. The catalytic apparatus of alpha/beta hydrolases typically involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine, and often the mechanism involves a nucleophilic attack on a carbonyl carbon atom. Lipases are glycerol ester hydrolases that act on acylglycerols to liberate fatty acids and glycerol. Lipases can be divided into two main groups: (i) Carboxylesterases (EC 3.1.1.1) and (ii) “true” lipases (EC 3.1.1.3), which differ in several biochemical features. Staphylococcus lipases constitute a distinctive subfamily of microbial lipases that was named the lipase subfamilyI.5. All lipases are members of the α/β hydrolase fold super-family. Also, lipases share a conserved active site signature, the Gly-Xaa-Ser-Xaa-Gly motif